poly mono adp ribose e6f6a antibody  (Cell Signaling Technology Inc)

Journal: Cell death and differentiation

Article Title: STING directly interacts with PAR to promote apoptosis upon acute ionizing radiation-mediated DNA damage.

doi: 10.1038/s41418-025-01457-z

Figure Lengend Snippet: Fig. 3 The STING response to DNA damage after IR is associated with PAR-PARP1. A Confocal images of the interactions between PARP1- Alex647 and STING-GFP via a Zeiss Elyra-7 microscope. B Quantification of the interaction between PARP1-Alex647 and STING-GFP via ImageJ software. Colocalization analysis was carried out via Manders’ colocalization coefficient (MCC) method. C Evaluating the association of STING with PARP1 in BMDMs. The cell lysates were immunoprecipitated with anti-STING beads, followed by immunoblotting with the indicated antibodies. D Coimmunoprecipitation and immunoblotting of the interaction of STING with PAR in THP-1 cells subjected to 30 Gy IR for 6 h. The cell lysates were immunoprecipitated with anti-STING beads, followed by immunoblotting with the indicated antibodies. E, F Microscopy images of PAR-Alex647 and STING-GFP interactions captured with ZEISS Elyra-7 (E) and quantified with ImageJ software (F). G Validation of the ability of PLA to detect the proximity between STING and PAR or PARP1 in MDA-MB-231 cells. Nuclei were stained with DAPI (blue); PLA was performed for STING and PAR or PARP1 (red). CON, non-irradiated. H, I The binding of STING and PAR in vitro was analyzed by nondenaturing polyacrylamide gel electrophoresis and immunoblotting with anti-STING (H) and anti-PAR (I) antibodies. J THP-1 cells were transfected with the indicated concentrations of PAR and cGAMP via Lipofectamine. PAR (0.2 μM) induced the phosphorylation of STING. The data are presented as the means ± SEMs (unpaired Student’s t test; ***, p < 0.001).

Article Snippet: The rabbit antibodies anti-PARP (46D11) antibody (9532) and anti-PAR (E6F6A) antibody (83732) were purchased from Cell Signaling Technology (Denver, MA).

Techniques: Microscopy, Software, Immunoprecipitation, Western Blot, Biomarker Discovery, Staining, Irradiation, Binding Assay, In Vitro, Polyacrylamide Gel Electrophoresis, Transfection, Phospho-proteomics